B8レポート

Intercalating fluorescence dye YOYO-1 prevents the folding transition in giant duplex DNA

義永 那津人

Abstract:

Recently, it has become clear that, with the addition of polyamines, giant DNA molecules of size greater than 10 kbp exhibit all-or-none switching between elongated coil and folded compact states. Here, the effects of the intercalating fluorescent labeling dye, YOYO-1, and the minor-groove binding fluorescent labeling dye, DAPI, on the folding transition of single giant T4 DNA (166 kbp) induced by spermidine 3+ were examined, by use of the experimental technique of single molecular chain observation with fluorescence microscopy. It is found that the intercalating dye, YOYO-1, markedly prevents the folding transition, whereas the minor-groove binding dye, DAPI, exhibits negligible effect on the folding transition. This action of YOYO-1 is discussed in relation to the biological effect of intercalators.


全文:

本文 report.pdf (33.4KB)
図1 fig1.pdf (549KB)
図2 fig2.pdf (332KB)
図3 fig3.pdf (328KB)
図4 fig4.pdf (14.9KB)



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